GET A CLEAR VIEW OF HOW THE STATE OF YOUR PROTEIN CHANGES

Use Labbot to automate and combine proven biophysical techniques in a new way. Generate reliable data easily. Discover new experiments and expand your scientific horizons.

Alexander K. Buell
DTU

“It really has potential to add value to studies of phase transitions, with it we can map the behavior with a high level of detail”

Prof. Sara Snogerup Linse
Lund University

“One of the best features is that we can track the reaction with multiple read­outs from the same sample. It really gives a few extra layers of insights.”

A portrait of Labbot customer Cedric Dicko
Prof. Cedric Dicko
Lund University

“It does everything we need when it comes to protein characterization”

Operator Independence

Different operators – same result

Minimal running costs

Labbot uses no proprietary consumables

Tiny footprint

Not bigger than a shoe box

Labbot is used by leading research groups

Applications

What can you investigate with Labbot?

The list of featured applications is long and ever growing. Here are some of the most used ones.

Amyloid Aggregation
Liquid-Liquid Phase Separation
Protein/ligand interactions
Thermal Stability
pH Stability
Metal Ion Binding

And many more ...

Labbot is not a tool to do just one thing. We offer a platform of core capabilities and the freedom to put them together to fit the experiment you want to do. We are excited to see what you will come up with.

A photo of Labbot next to a computer and a user.

Labbot is your digital lab partner – designed to automate and refine difFicult experiments

Labbot allows you to control and investigate any liquid sample you can fit into a standard cuvette, combining simultaneous UV-vis Absorbance, Fluorescence, Static light scattering and a pH- or Conductivity meter for a complete view of your sample.

The nanoliter titration system and state-of-the-art temperature control allow you to control the state of your sample with high precision.

Take your sample to a well defined state:

Automatic Titration

Minimum addition
20 nl
Time Resolution
≤ 500 ms (depending on integration time)

Temperature Control

12–87 C with 0.2 C accuracy (to NIST traceable reference)

4–87 C with drying gas

Mixing

Magnetic stirrer with adjustable speed

Sample format

Standard Optical Cuvette
Base 12.5x12.5 mm. Z-height 8 mm
Minimum Volume
50–900 μl,  depending on application
Maximum Volume
3 ml

and make simultaneous measurements of:

UV-Vis Absorbance

Light Source
Xenon flash
Spectral Range
225-770 nm, 0.5 nm optical resolution
Linear Range
0.01–1.5 AU

Fluorescence

Excitation
Excitation Xenon flash with up to 10 filters
Spectral Range
290-840 nm, 11 nm optical resolution

Static Light Scattering

Laser Based
Red laser recorded at 90°
Detector
High sensitivity photo diode

pH- or Conductivity Meter

Integrate external meter with instrument software
Real-time Feedback

Get beautiful data

Why you should get a Labbot

Operator Independent

The results are reliable, accurate, and repeatable, with no variation between individual operators. Little training needed to get started.

You’re in Control

No black box experiments. Labbot saves raw data in an accessible state, so you can get the full picture of your experiment no matter what analysis you undertake.

Minimal Running Costs

Labbot uses no proprietary consumables. Run as many experiments as you want without worrying about access to materials.

Customize Your Experiment

With powerful software and a team that responds quickly, we make sure that you can design your experiments the way you want to.

Combine Modes of Measurement

Get the full picture by combining up to four different modes of measurement in the same sample.

Tiny footprint

Four modes of measurement and complete sample control packed into an instrument the size of a shoe box.

Reclaim Your Time

While Labbot is busy executing your instructions you could already be thinking about your next experiment.

Improve Data Quality

Continuous automatic measurements means denser data points, with accurate and reliable concentrations and timing.

Support and Service that Care

If you are experiencing technical difficulties or have questions about how to make a specific application work, our team is here to support you.

Why you will love your Labbot

An illustration of Cedric Dicko with a Labbot that enables new experiments

It will enable new experiments ...

Automation can be much more than a simple quality-of-life improvement. The enhanced reproducibility, precise control and freed-up time will also bring experiments that were previously too challenging into the realm of the possible.

... that will spark new ideas in your mind ...

Add new measurement techniques to your existing protocols? Take an old measurement and expand it across temperature or concentration? Automate a tedious procedure and multiply your output?

An illustration of Cedric Dicko with new ideas sparking in his mind.
An illustration of Cedric Dicko receiving a grand award from Sara Snogerup Linse

... and push your research to new frontiers.

You deserve the best tools available for your science. We are excited to see what new possibilities you will unlock with your Labbot.

A photo of Sara Snogerup Linse smiling next to a prior Labbot.
Professor of physical chemistry at Lund University, Sara Snogerup Linse is a proud owner (and often the operator) of four Labbots.
Client Case

Just as it did for
Sara Snogerup Linse

An early adopter since the first version, Sara and her group have put Labbot to work in projects ranging from calcium binding to the role of electrostatics in protein aggregation.

In an ongoing study she is following dissociation kinetics by rapidly diluting samples and recording a combination of FRET and light scattering. With Labbot it has been convenient to repeat the experiment at different temperatures to explore the thermodynamics of the system.

ARTICLE

Charge Regulation during Amyloid Formation of α-Synuclein

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ARTICLE

An aggregation inhibitor specific to oligomeric intermediates of Aβ42 derived from phage display libraries of stable, small proteins

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"One of the best features is that we can track the reaction with multiple readouts from the same sample. It really gives a few extra layers of insights."

Discover if Labbot is a good fit for your lab

Talk to our application specialist to find out how your lab can benefit from investing in a Labbot.

Featured Articles

ANALYTICAL BIOCHEMISTRY, 2009

Precise detection of pH inside large unilamellar vesicles using membrane-impermeable dendritic porphyrin-based nanoprobes

Thom Leiding and co-authors

This 2009 paper serves as the origin story of Labbot. Faced with the need to characterize the response of a pH sensitive probe with very high precision, Thom decided to automate the task. The device featured a CCD detector with the capacity to automatically record absorbance spectra as a set of computer controlled syringe pumps added small volumes of acid or base.It was clear already from the beginning that this method not only reduced the hassle of making these measurements, but also greatly improved the data density, quality and precision.

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THE PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES (PNAS), 2022

An aggregation inhibitor specific to oligomeric intermediates of Aβ42 derived from phage display libraries of stable, small proteins

LINSE AND CO-AUTHORS

The authors identified and characterised peptide inhibitors of amyloid aggregation. The interactions between the inhibitors and amyloid fibrils are confirmed using several complementary methods, including Förster Resonance Energy Transfer (FRET). By labelling the interaction partners with FRET-compatible Alexa dyes and following their fluorescence intensities they could monitor the interactions throughout the entire reaction.

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ANALYTICAL CHEMISTRY, 2021

Capillary flow experiments (Capflex) for thermodynamic and kinetic characterization of protein LLPS at high throughput

Stender and co-authors

This paper describes a method to study liquid-liquid phase separation (LLPS). In order to determine the onset of droplet formation and identify optimal starting conditions for their experiments, the authors followed light scattering and fluorescence as functions of temperature.

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Journal of the American Chemical Society, 2021

Charge Regulation during Amyloid Formation of α-Synuclein

Pálmadóttir and co-authors

This paper focuses on the interplay between electrostatics and aggregation in an amyloid system. Rather than changing the pH by titration and recording the response, the authors follow a reaction in an unbuffered system and record the resulting pH change. They also follow the reaction using fluorescence readout and static light scattering in order the pH response to the aggregation reaction.

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Personal Support & Service

We are proud to work with our users to facilitate great science. If you have any technical problems or have questions about how to make a specific application work, our small but dedicated team is here to help.

A photo of Thom and Mattias doing service of a Labbot.
Our Story

It all started about 15 years ago ...

... I was a fresh Ph.D. student in Biochemistry, yearning to plunge into science. The goal of the project I was assigned was to understand what made a specific virus protein so important for the virus to spread. 

A collage of photos displaying Labbot's history.

The idea was to reconstitute the protein into artificial cell membranes and measure the flow of ions through it. Long story short, me and my supervisor Sindra decided to use a proton gradient as a driving force and a pH-sensitive dye as a reporter.

There was a tricky thing with this method. It would require me to perform an exact pH calibration of the dye for every batch of proteoliposomes we made. Although the principle of pH titration is quite simple, anyone who has made one manually knows it is difficult especially if you want equidistant data points. 

The Team

Mattias Törnquist, PhD

Application Scientist
Mattias is in charge of making sure that you can get the best science out of Labbot. He supports customers in their daily use, works on interface and methods and participates in scientific collaborations. He has a PhD in Biochemistry and a master’s in Engineering Nano Science.

Maximilian Leiding

Communication & Design
It is Maximilian’s job to get the word out about Labbot. He collaborates with the team to enhance all of our channels, from the website to slideshows. He has experience working for several start-ups and has studied digital design and strategic communication.

Thom Leiding, PhD

CEO & INVENTOR
During his PhD, Thom invented the first version of Labbot. His background in software, computer engineering, and electronics, along with his many years of experience as a scientist, give him an edge when it comes to inventing highly usable laboratory instruments. Thom has a PhD in Biochemistry.

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